Cullin 2 Antibody Summary
| Immunogen |
Peptide with sequence C-QYIERSQASADE corresponding to C-Terminus according to NP_003582.2.
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| Epitope |
QYIERSQASADE – C Terminus
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
CUL2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 100 kDa band observed in NIH3T3 lysates (calculated MW of 87.0 kDa band according to human NP_003582.2 and of 86.9 kDa band according to mouse NP_083678.2).
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for Cullin 2 Antibody
- CUL-2
- cullin 2
- cullin-2
- MGC131970
Background
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
