Dystroglycan Antibody Summary
| Immunogen |
Peptide with sequence C-HVGKHEYFMHATDK corresponding to internal region according to NP_004384.4.
|
| Epitope |
C-HVGKHEYFMHATDK
|
| Specificity |
This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
|
| Clonality |
Polyclonal
|
| Host |
Goat
|
| Gene |
DAG1
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
WB: Approx. 100 kDa band observed in human skeletal muscle lysates (calculated MW of 97.4 kDa band according to NP_004384.4). Approx. 80 kDa band observed in mouse and rat skeletal muscle lysates (calculated MW of 96.9 kDa band according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan.
|
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Canine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for Dystroglycan Antibody
- A3a
- AGRNR
- DAG1
- Dag-1
- DAG156DAG
- dystroglycan 1 (dystrophin-associated glycoprotein 1)
- Dystroglycan
- Dystrophin-associated glycoprotein 1
Background
Dystroglycan (also known as dystrophin-associated glycoprotein 1) is a member of the dystroglycan family that contains the WWW binding motif PPxY. This protein has alpha and beta subunits with approximate molecular weights of 156 kD and 43-50 kD, respectively. The dystroglycan beta subunit is an integral membrane protein, the alpha subunit is a ubiquitously expressed extracellular protein. Muscle and non-muscle isoforms differ by carbohydrate moieties (not protein sequence). Dystroglycan functions as an adhesion molecule responsible for interactions between extracellular matrix and the subsarcolemmal cytoskeleton. Dsytroglycan binds to lamin in the matrix and dystrophin in the cytoskeleton; phosphorylation regulates the association of interacting proteins. Dystroglycan binds to utrophin when unphosphorylated; c-Src, Fyn, Csk, NCK, and SHC when phosphorylated. Dystroglycan interacts with dystrophin through the WW domain. The Poly6171 antibody recognizes human phosphorylated dystroglycan (Tyr893) and has been shown to be useful for Western blotting.
