Hrk Antibody Summary
| Immunogen |
Antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
HRK
|
| Purity |
Immunogen affinity purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
||
| Application Notes |
Hrk antibody can be used for the detection of Hrk by Western blot at 2. 5 – 5 mg/ml.
|
||
| Theoretical MW |
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control Peptide |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for Hrk Antibody
- activator of apoptosis harakiri
- activator of apoptosis Hrk
- BCL2-interacting protein
- BH3-interacting domain-containing protein 3
- BID3
- death protein 5
- DP5
- HARAKIRI
- harakiri, BCL2 interacting protein (contains only BH3 domain)
- harakiri, BCL2-interacting protein (contains only BH3 domain)
- Hrk
- Neuronal death protein DP5
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
