Clathrin light chain Antibody (CON.1) [DyLight 680]

Clathrin light chain Antibody (CON.1) [DyLight 680] Summary

Immunogen	Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain.
Localization	Cell Membrane and Cytoplasmic
Specificity	Monoclonal Anti-Clathrin Light Chain reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. The product is useful in immunoblotting 2-4 (two doublets at 25-36 kDa, a doublet at approximately 56 kDa, and possibly also additional lower and higher molecular weight bands), immunoprecipitation, immunocytochemistry (4% paraformaldehyde- 0.5% Triton X-100 fixation), and immunoaffinity purification. Cross-reactivity has been observed with human, bovine, rat, mouse, and yeast.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	CLTA
Purity	Protein A or G purified
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Applications/Dilutions

Dilutions	Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cross-reacts S. cerevisiae. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.