GATA-4 Antibody (Evan) Summary
| Immunogen |
The immunogen for this antibody was Gata4.
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| Isotype |
IgG2a Kappa
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
GATA4
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
This Evan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2ug/ml for western blot analysis.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Aqueous, may contain carrier protein/stabilizer.
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for GATA-4 Antibody (Evan)
- GATA binding protein 4
- GATA4
- GATA-4
- GATA-binding factor 4
- GATA-binding protein 4
- MGC126629
- transcription factor GATA-4
Background
The Evan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The Evan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.
