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Product: Levobunolol (hydrochloride)

Caspase Colorimetric Assay Kit Summary

Description
Caspase-1/ICE Activity Colorimetric Assay Kit: Simple & Convenient colorimetric Assay to Measure Caspase-1/ICE Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.

  • Detection method- Absorbance (400 or 405 nm)
  • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit Type
Colorimetric Assay Kit

Applications/Dilutions

Application Notes
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 2X Reaction Buffer
  2. Cell Lysis Buffer
  3. DTT (1 M)
  4. Dilution Buffer
  5. YVAD-pNA (4 mM)

PMID: 7720709

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Author: premierroofingandsidinginc