MIF Antibody Summary
| Immunogen |
Synthetic peptide surrounding amino acid 67 of human MIF (CAG46452).
|
| Specificity |
MIF
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
MIF
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western blotting (0.5-4 ug/ml) and immunoprecipitation (5-10 ug/ml). However, the optimal conditions should be determined individually.
|
| Theoretical MW |
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
May cross react with Human, pig, and bovine
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
|
| Preservative |
0.01% Thimerosal
|
| Concentration |
0.2 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for MIF Antibody
- EC 5.3.2.1
- EC 5.3.3.12
- GIFmacrophage migration inhibitory factor
- GLIF
- Glycosylation-inhibiting factor
- L-dopachrome isomerase
- L-dopachrome tautomerase
- macrophage migration inhibitory factor (glycosylation-inhibiting factor)
- MIF
- MMIF
- Phenylpyruvate tautomerase
Background
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of microphages. Recently, MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF also plays a critical role in the host control of inflammation and immunity.
