Product: Ginkgolic Acid (C13:2)
Lactate Dehydrogenase Antibody Summary
| Immunogen |
Full length LDH isolated from rabbit muscle.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Goat
|
| Gene |
LDHB
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western blotting (1:500-2500). However, the optimal conditions should be determined individually. The antibody recognizes ~36 kDa LDH. HeLa cell lysate can be used as a positive control.
|
| Theoretical MW |
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
|
| Preservative |
0.01% Thimerosal
|
| Concentration |
5 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for Lactate Dehydrogenase Antibody
- EC 1.1.1
- EC 1.1.1.27
- lactate dehydrogenase B
- LDH heart subunit
- LDH-B
- LDH-H
- L-lactate dehydrogenase B chain
- Renal carcinoma antigen NY-REN-46
- TRG-5
Background
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types – M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream). Suitable for immunoblotting (western or dot blot), ELISA, conjugation and most immunological methods requiring high titer and specificity.
