Patched 1/PTCH Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC216999 and based on accession number NM_000264. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b, mRNA.
|
| Gene |
PTCH1
|
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
160.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
|
Lysate Details for Patched 1/PTCH
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Patched 1/PTCH Overexpression Lysate (Native)
- BCNS
- BCNSFLJ42602
- FLJ26746
- HPE7
- mes
- NBCCS
- patched (Drosophila) homolog
- Patched 1
- patched homolog (Drosophila)
- patched homolog 1 (Drosophila)
- patched homolog 1
- protein patched homolog 1
- PTC
- ptc1
- PTCH protein +12b
- PTCH protein +4
- PTCH protein -10
- PTCH
- PTCH1
- PTCH11
Background
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq]. Transcript Variant: This variant (1b) encodes the longest isoform (L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
