Product: D-Lys(Z)-Pro-Arg-pNA (diacetate)
KISS1 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC206859 and based on accession number NM_002256.
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| Epitope |
C-terminal DDK tag
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| Specificity |
Homo sapiens KiSS-1 metastasis-suppressor (KISS1), mRNA.
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| Gene |
KISS1
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
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|
| Theoretical MW |
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
RIPA buffer
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Lysate Details for KISS1
| Type |
Overexpression
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| Protein State |
Native
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Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for KISS1 Overexpression Lysate (Native)
- KiSS-1 metastasis-suppressor
- KiSS1
- KiSS-1
- kisspeptin
- Kisspeptin-1
- malignant melanoma metastasis-suppressor
- metastasis-suppressor KiSS-1
- Metastin
- MGC39258
Background
This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
