Cytochrome P450 26B1 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC221075 and based on accession number NM_019885. The protein contains a C-terminal DDK tag.
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| Specificity |
Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1), mRNA.
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| Gene |
CYP26B1
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
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|
| Theoretical MW |
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
RIPA buffer
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Lysate Details for Cytochrome P450 26B1
| Type |
Overexpression
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| Protein State |
Native
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Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Cytochrome P450 26B1 Overexpression Lysate (Native)
- CYP26A2
- Cytochrome P450 26A2
- cytochrome P450 26B1
- cytochrome P450 CYP26B1 variant 2
- Cytochrome P450 retinoic acid-inactivating 2
- cytochrome P450 retinoid metabolizing protein
- cytochrome P450, family 26, subfamily B, polypeptide 1
- Cytochrome P450RAI-2
- DKFZp686G0638
- EC 1.14
- MGC129613
- P450RAI2
- P450RAI-2
- Retinoic acid-metabolizing cytochrome
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and the synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene is involved in the specific inactivation of all-trans-retinoic acid to hydroxylated forms, such as 4-oxo-, 4-OH-, and 18-OH-all-trans-retinoic acid. [provided by RefSeq].
