Glucosylceramidase/GBA Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC201614 and based on accession number NM_001005741. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 2, mRNA.
|
| Gene |
GBA
|
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
55.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
|
Lysate Details for Glucosylceramidase/GBA
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Glucosylceramidase/GBA Overexpression Lysate (Native)
- Acid beta-glucosidase
- Alglucerase
- beta-glucocerebrosidase
- D-glucosyl-N-acylsphingosine glucohydrolase
- EC 3.2.1.45
- GBA
- GBA1
- GC
- GCB
- GLUC
- glucosidase, beta, acid
- glucosidase, beta; acid (includes glucosylceramidase)
- Glucosylceramidase
- Imiglucerase
- lysosomal glucocerebrosidase
Background
This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5 UTR, compared to variant 4. All transcript variants of this gene encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
