ER alpha/NR3A1 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC213277 and based on accession number NM_000125. The protein contains a C-terminal DDK tag.
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| Specificity |
Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1, mRNA.
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| Gene |
ESR1
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
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|
| Theoretical MW |
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
RIPA buffer
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Lysate Details for ER alpha/NR3A1
| Type |
Overexpression
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| Protein State |
Native
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Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for ER alpha/NR3A1 Overexpression Lysate (Native)
- ER alpha
- ER
- Era
- ER-alpha
- ESR1
- ESRESRA
- Estradiol receptor
- estrogen receptor 1
- estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
- estrogen receptor alpha delta 4 +49 isoform
- estrogen receptor alpha delta 4*5,6,7*/654 isoform
- estrogen receptor alpha
- estrogen receptor
- NR3A1
- NR3A1DKFZp686N23123
- Nuclear receptor subfamily 3 group A member 1
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5 UTRs and use different promoters. [provided by RefSeq]. Transcript Variant: This variant (1) uses the most proximal promoter. Variants 1-4 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
