Cyclin B1 Antibody (V143.1) Summary
| Immunogen |
His tagged cyclin B1 (hamster) expressed in bacteria.
|
| Epitope |
Within a region spanning the carboxy region.
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| Localization |
Nuclear
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| Marker |
Mitosis Marker
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| Isotype |
IgG1
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CCNB1
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
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|
Applications/Dilutions
| Dilutions |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for Cyclin B1 Antibody (V143.1)
- CCNB
- CCNB1
- Cyclin B1
- G2/mitotic-specific cyclin B1
- G2/mitotic-specific cyclin-B1
Background
In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
