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Product: Lifitegrast

Zic2 Antibody Summary

Immunogen
Peptide with sequence C-HSGLSSNFNEWY corresponding to C-Terminus according to NP_009060.2.
Specificity
This antibody is expected to NOT cross-react with other ZIC proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZIC2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 – 2 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: In transfected RWPE1 transiently expressing human ZIC2-Myc a band of approx. 65 kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0 kDa band according to NP_009060.2 and 58.6 kDa band for the ZIC2-Myc protein, provided by Nathan Bowen and Maryam Boseman, Clark Atlanta University, GA, USA. An approx. 75 kDa band in human brain (cerebellum and cerebral cortex) lysates.
Publications
Read Publication using NBP2-27506.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Zic2 Antibody

  • HPE5Zic family member 2 (odd-paired Drosophila homolog)
  • Zic family member 2 (odd-paired homolog, Drosophila)
  • Zinc finger protein of the cerebellum 2
  • zinc finger protein ZIC 2

PMID: 17075569

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Author: premierroofingandsidinginc