Neutrophil Elastase/ELA2 Antibody Summary
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human Neutrophil elastase. The exact sequence is proprietary.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
ELANE
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
|
| Preservative |
0.01% Thimerosal
|
| Concentration |
1 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for Neutrophil Elastase/ELA2 Antibody
- Bone marrow serine protease
- EC 3.4.21
- EC 3.4.21.37
- ELA2
- ELA2granulocyte-derived elastase
- ELANE
- elastase 2, neutrophil
- elastase, neutrophil expressed
- Elastase-2
- GE
- HLEelastase-2
- HNE
- Human leukocyte elastase
- Leukocyte Elastase
- Medullasin
- NE
- Neutrophil Elastase
- PMN elastase
- PMN-E
- polymorphonuclear elastase
- SCN1
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Elastase 2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the proteins release from activated neutrophils. Elastase 2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq]
