Recombinant Human FKBP12 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 108 of Human FKBP12
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
FKBP1A
|
| Purity |
>95% pure by SDS-PAGE
|
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method)
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 14.1 kDa (128aa), confirmed by MALDI-TOF.
Assay |
| Theoretical MW |
14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Human.
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM DTT, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human FKBP12 Protein
- 12 kDa FKBP
- EC 5.2.1.8
- FK506 binding protein 1A, 12kDa
- FK506-binding protein 1A
- FKBP1
- FKBP12
- FKBP12C
- FKBP12FKBP12-Exip3
- FKBP-12T-cell, 12-kD
- FKBP1a
- FKBP-1A
- peptidyl-prolyl cis-trans isomerase FKBP1A
- PKC12
- PPIase FKBP1A
- PPIASE
- protein kinase C inhibitor 2
- rotamase
Background
FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
