Recombinant Human Sulfatase Modifying Factor 1/SUMF1 Protein Summary
| Description |
A denatured recombinant protein with a His-tag corresponding to amino acids 91 to 374 of Human Sulfatase Modifying Factor 1/SUMF1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
SUMF1
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl, pH8.0, 2M urea, 20% glycerol, 2mM DTT
|
| Purity |
>85%, by SDS-PAGE
|
Notes
The purity of this protein is > 85% by SDS-PAGE. Molecular weight is 34.1kDa (304aa)
Alternate Names for Recombinant Human Sulfatase Modifying Factor 1/SUMF1 Protein
- AAPA3037
- EC 1.8.99.-
- FGE1
- FGEC-alpha-formylglycine-generating enzyme 1
- FGly-generating enzyme
- MGC131853
- MGC150436
- sulfatase modifying factor 1
- sulfatase-modifying factor 1
- SUMF1
- UNQ3037
Background
SUMF1 (Sulfatase-modifying factor 1) belongs to the SUMF family. SUMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant human SUMF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
